TMB X-tra
TMB (3, 3′, 5, 5′-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).
TMB ONE characteristics are:
- “The Gold Standard” used across the industry
- Incredibly stable and robust
- Aqueous based – no organic solvents (REACH Annex XVII compliant)
- Low lot-to-lot variation
- Suitable for use in electrochemical assays
For traceable pipetting, we recommend using pink colored TMB ONE Prestained. The pink indicator disappears after addition of the stop solution.
Specifications
- Available sizes: 100 mL, 500 mL, 1 L , bulk
- Format: Ready-to-use (RTU)
- Storage: 2-8⁰C in the dark
- Stability: 4 years
- Development time: 5-30 minutes
- Stop solution: 0.2 M H₂SO₄
