TMB X-tnd

TMB (3, 3′, 5, 5′-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm.

TMB X-tnd is developed to support assays, which require low detection limits while supporting OD readings of highly positive samples – with no precipitation of the TMB substrate, at up to 5.8 mAbs within 60 minutes.

TMB X-tnd characteristics are:

• Best dynamic range
• No precipitation
• Aqueous based – no organic solvents (REACH Annex XVII compliant)
• Low lot-to-lot variation
• Suitable for use in electrochemical assays

Specifications

• Available sizes: 100 mL, 500 mL, 1 L, bulk
• Format: Ready-to-use (RTU)
• Storage: 2-8⁰C in the dark
• Stability: 4 years
• Development time: 5-30 minutes
• Stop solution: 0.35 M H₂SO₄