TMB PLUS2® (& prestained)

TMB (3, 3′, 5, 5′-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).

TMB PLUS2® characteristics are:

• Medium sensitivity
• Very low background
• Aqueous based – no organic solvents (REACH Annex XVII compliant)
• Low lot-to-lot variation
• Suitable for use in electrochemical assays

For traceable pipetting, we recommend using pink colored TMB PLUS2® Prestained. The pink indicator disappears after addition of the stop solution.

Specifications

• Available sizes: 100 mL, 500 mL, 1 L, bulk
• Format: Ready-to-use (RTU)
• Storage: 2-8⁰C in the dark
• Stability: 4 years
• Development time: 5-30 minutes
• Stop solution: 0.2 M H₂SO₄