BCIP/NBT

Precipitating AP Substrate

BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/ Nitro Blue Tetrazolium) liquid ready-to-use substrate is a highly active and stable formulation utilized for colorimetric detection of Alkaline Phosphatase (AP) activity in membrane assays. Positive reactions form an intense blue/purple precipitate at the site of the reaction. The color develops when AP catalyzes the dephosphorylation of BCIP and converts NBT to insoluble blue/purple NBT formazan. The intense blue/purple precipitate is very stable and resists fading when exposed to light.

The product is available in bulk quantities and as customized solutions.

Specifications

• Available sizes: 100 mL, 500 mL, 1 L, bulk
• Format: Ready-to-use (RTU)
• Storage: 2-8⁰C in the dark
• Stability: 4 years
• Development time: 5-30 minutes